Notable Gleaming Toxic Jam features a single song broken into its four separate tracks (drums, keyboard, guitar, and bass) that are rapidly approaching extinction. The player has a series of minigame challenges ("trivia" questions, color matching, and don't-touch-the-wall mazes). For each minigame the player beats, they're given points they can add to a single track to keep it alive.

The player loses if all four tracks go extinct. The player wins if they make it through the entire song.